7BYU

Crystal structure of Acidovorax avenae L-fucose mutarotase (apo form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	69.465, 69.465, 69.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.415 - 2.206
R-factor	0.2181
Rwork	0.217
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x8d
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.200	2.210
Rmeas	0.085	0.843
Rpim	0.037	0.372
Number of reflections	18794	1637
<I/σ(I)>	10.6	1.8
Completeness [%]	99.7	98
Redundancy	5.2	2.58
CC(1/2)	0.996	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8 M potassium sodium tartrate, 0.1 M Tris-HCl pH 8.5, 0.5% PEGMME 5000