7BYK
Crystal structure of the Legionella pneumophila LegK7 effector kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.996, 74.198, 201.585 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.220 - 2.650 |
R-factor | 0.2071 |
Rwork | 0.205 |
R-free | 0.25260 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.680 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.114 | 0.423 |
Number of reflections | 30648 | 1478 |
<I/σ(I)> | 19 | 3.2 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 6.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | PEG 600, imidazole, calcium acetate |