7BYF
The crystal structure of mouse ORF10-Rae1-Nup98 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-10-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.397, 150.630, 101.618 |
| Unit cell angles | 90.00, 111.03, 90.00 |
Refinement procedure
| Resolution | 48.903 - 2.500 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.23310 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.981 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.903 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.155 | 0.597 |
| Number of reflections | 63997 | 12416 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M di-Sodium tartrate, 20% PEG 3350, pH 7.5 |
