7BXW

Crystal structure ofF RTT109 FROM Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-04-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.975
Spacegroup name	P 1 21 1
Unit cell lengths	51.479, 69.564, 54.590
Unit cell angles	90.00, 114.70, 90.00

Refinement procedure

Resolution	49.593 - 1.776
R-factor	0.164712564487
Rwork	0.163
R-free	0.20118
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.393
Data reduction software	HKL-2000
Data scaling software	HKL-2000 (V1.0)
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.800
High resolution limit [Å]	1.770	4.800	1.770
Rmerge	0.078	0.059	0.298
Rmeas	0.084	0.063	0.321
Rpim	0.030	0.022	0.117
Total number of observations	252953
Number of reflections	33152	1740	1599
<I/σ(I)>	5.9
Completeness [%]	98.4	99.4	96.1
Redundancy	7.6	7.6	7.1
CC(1/2)		0.996	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289.15	0.1 M Bis-Tris pH 6.0, 15-20% PEG 3350