7BX0

Crystal structure of RTT109 from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	193.15
Detector technology	CCD
Collection date	2014-11-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	51.356, 69.919, 54.682
Unit cell angles	90.00, 115.08, 90.00

Refinement procedure

Resolution	25.650 - 1.500
R-factor	0.182
Rwork	0.182
R-free	0.19290
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.650	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	410518	410518
<I/σ(I)>	5.615
Completeness [%]	97.9
Redundancy	7.5
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	0.1 M Bis-Tris pH 6.0, 15~20% PEG 3350