7BWY
Crystal structure of ice-binding protein from an Antarctic ascomycete, Antarctomyces psychrotrophicus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.311, 92.311, 222.669 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.725 - 2.020 |
| R-factor | 0.1808 |
| Rwork | 0.179 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7bwx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.081 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.160 | 2.060 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.082 | 1.654 |
| Rmeas | 0.085 | 1.698 |
| Rpim | 0.382 | |
| Number of reflections | 72989 | 4427 |
| <I/σ(I)> | 21.1 | 2.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 19.8 | 19.6 |
| CC(1/2) | 1.000 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Bis-Tris pH 7.0, 4.0 M ammonium acetate |
