7BWU

Restructuring hemagglutinin-neuraminidase (HN) of Newcastle disease virus produced from Oryza sativa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	83.880, 142.070, 191.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.863 - 1.870
R-factor	0.1682
Rwork	0.167
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t1e
RMSD bond length	0.007
RMSD bond angle	0.919
Data reduction software	xia2
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.530	1.930
High resolution limit [Å]	1.870	1.870
Rmerge	0.113
Rmeas	0.118
Rpim	0.032
Number of reflections	188493	18656
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	60% Tacsimate