7BW9
Crystal structure of Serine acetyltransferase isoform 3 in complex with cysteine from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-04-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.323, 76.307, 96.726 |
| Unit cell angles | 90.00, 95.52, 90.00 |
Refinement procedure
| Resolution | 39.540 - 2.131 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p47 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_3352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rpim | 0.053 | 0.280 |
| Number of reflections | 48708 | 2424 |
| <I/σ(I)> | 15.3 | 2.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 5.2 | 4.6 |
| CC(1/2) | 0.950 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 0.1 M Hepes pH 7.5, 0.2 M KSCN, 0.2 M KBr, 25 % PEG 3350 |
