7BW2
Crystal Structure of Cyanobacterial PSI Monomer from T.elongatus at 6.5 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 187.029, 187.029, 233.805 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.570 - 6.500 |
| R-factor | 0.413 |
| Rwork | 0.409 |
| R-free | 0.48600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.570 | 6.730 |
| High resolution limit [Å] | 6.500 | 6.500 |
| Rmerge | 0.055 | 0.335 |
| Number of reflections | 9572 | 949 |
| <I/σ(I)> | 29.58 | 7.39 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 10.7 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277.15 | no salt |
