7BVX

Crystal structure of C-terminal fragment of pilus adhesin SpaC from Lactobacillus rhamnosus GG-Iodide soaked

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.42310
Spacegroup name	I 2 2 2
Unit cell lengths	60.045, 114.919, 122.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.460 - 3.360
R-factor	0.30677
Rwork	0.305
R-free	0.34573
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3y
RMSD bond length	0.010
RMSD bond angle	1.930
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.530	3.410
High resolution limit [Å]	3.350	3.350
Rmerge	0.055	0.860
Rpim	0.016	0.240
Number of reflections	6345	324
<I/σ(I)>	34.7	2.3
Completeness [%]	100.0	100
Redundancy	12.6	13.4
CC(1/2)	0.990	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	2M Lithium Sulfate