7BVW

Crystal structure of the RING-H2 domain of Arabidopsis RMR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2018-01-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	74.431, 23.648, 75.699
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	32.230 - 2.100
R-factor	0.1815
Rwork	0.179
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v3k
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.720	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.098	0.213
Rmeas	0.116	0.253
Rpim	0.061	0.135
Total number of observations	27884
Number of reflections	7849	650
<I/σ(I)>	9
Completeness [%]	98.5	97.4
Redundancy	3.6	3.4
CC(1/2)	0.992	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	289	0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 20% w/v Polyethylene glycol 3,350