7BVW
Crystal structure of the RING-H2 domain of Arabidopsis RMR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-01-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.431, 23.648, 75.699 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 32.230 - 2.100 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v3k |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.720 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.213 |
| Rmeas | 0.116 | 0.253 |
| Rpim | 0.061 | 0.135 |
| Total number of observations | 27884 | |
| Number of reflections | 7849 | 650 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 98.5 | 97.4 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.992 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.2 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 20% w/v Polyethylene glycol 3,350 |
