7BVR
DgpB-DgpC complex apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-10 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.516, 156.721, 158.931 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.890 - 2.600 |
| R-factor | 0.1924 |
| Rwork | 0.191 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7drd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.890 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.115 | 0.628 |
| Number of reflections | 67240 | 4482 |
| <I/σ(I)> | 13.3 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 6.6 |
| CC(1/2) | 0.997 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 313 | 100 mM Tris (pH 8.5), 24% PEG4000 |
