7BUZ
Crystal structure of Pennisetum glaucum monodehydroascorbate reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2019-01-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.572, 83.460, 132.872 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.670 - 2.288 |
R-factor | 0.1898 |
Rwork | 0.187 |
R-free | 0.24760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jci |
RMSD bond length | 0.008 |
RMSD bond angle | 0.990 |
Data reduction software | HKL-2000 |
Phasing software | BALBES (1.0.0) |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.675 | 2.327 |
High resolution limit [Å] | 2.288 | 2.288 |
Rpim | 0.066 | |
Number of reflections | 41184 | 41184 |
<I/σ(I)> | 2.18 | |
Completeness [%] | 100.0 | |
Redundancy | 15.7 | |
CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.2 M Sodium acetate trihydrate 0.1 M Sodium cacodylate trihydrate pH 6.5 30% w/v Polyethylene glycol 8,000 |