7BU5

Crystal Structure of Human SLX4 and MUS81

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97983
Spacegroup name	P 32 1 2
Unit cell lengths	59.118, 59.118, 86.425
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.040 - 1.800
R-factor	0.1705
Rwork	0.169
R-free	0.20600
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.008
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.033	0.021	0.254
Total number of observations	149691
Number of reflections	16333	1654	1624
<I/σ(I)>	19.8
Completeness [%]	99.6	96.1	100
Redundancy	9.2	8.2	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	277	25% w/v PEG 3350, 0.1M SPG (succinic acid: sodium dihydrogen phosphate: glycine, 2: 7: 7)