7BU0
Crystal structure of a OTU deubiquitinase in complex with Ub-PA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979176 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.930, 119.230, 84.530 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 48.615 - 2.430 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.25520 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.950 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 49112 | 3613 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.994 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289.15 | 0.1M Magnesium formate dihydrate 15% w/v PEG 3350 |
