7BTH

Mevo lectin- Native form-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-06-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	94.610, 168.220, 169.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.687 - 2.600
Rwork	0.295
R-free	0.30640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zno
RMSD bond length	0.012
RMSD bond angle	1.781
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.700	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.220
Number of reflections	41843	6030
<I/σ(I)>	7.9	2
Completeness [%]	100.0	100
Redundancy	9.5	14.7
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7	296	2.0 M ammonium citrate tribasic, 0.1 M bis tris propane pH 7.0