7BTG

Crystal structure of DARP, drosophila arginine phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2018-07-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	40.236, 62.196, 71.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.872 - 2.188
R-factor	0.2078
Rwork	0.203
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kk3
RMSD bond length	0.008
RMSD bond angle	1.051
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.188	2.188
Number of reflections	9684	795
<I/σ(I)>	39.9
Completeness [%]	98.2
Redundancy	5.3
CC(1/2)	0.992	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M ammonium phosphate monobasic, 100 mM sodium acetate trihydrate (pH 5.0), 10 mM calcium chloride