7BT8

Mevo lectin complex with mannotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-12-28
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	55.770, 157.220, 163.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.390 - 2.700
Rwork	0.210
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bsb
RMSD bond length	0.010
RMSD bond angle	1.663
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.390	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.190
Number of reflections	39930	5790
<I/σ(I)>	7	1.9
Completeness [%]	99.0	100
Redundancy	7
CC(1/2)	0.980	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	296	6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000