7BSN

Mevo lectin complex with 3alpha-mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 41 21 2
Unit cell lengths	169.748, 169.748, 195.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.018 - 2.600
Rwork	0.215
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bsb
RMSD bond length	0.012
RMSD bond angle	1.752
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.870	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.320
Number of reflections	88208	12714
<I/σ(I)>	7.4	2.8
Completeness [%]	100.0	100
Redundancy	24.4	24.1
CC(1/2)	0.740	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		296	6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000