7BSM

Mevo lectin complex with 2alpha-mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9767
Spacegroup name	P 41 21 2
Unit cell lengths	170.359, 170.359, 192.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.250 - 2.800
Rwork	0.227
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bsb
RMSD bond length	0.014
RMSD bond angle	1.890
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.250	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.220
Number of reflections	70202	10083
<I/σ(I)>	9	1.8
Completeness [%]	100.0	100
Redundancy	13.2
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		296	6%(v/v) Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25%(w/v) polyethylene glycol 4000