7BRN

Crystal structure of Atg40 AIM fused to Atg8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2017-03-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 32 2 1
Unit cell lengths	74.729, 74.729, 57.092
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.813 - 2.231
R-factor	0.2039
Rwork	0.203
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zpn
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.813	2.311
High resolution limit [Å]	2.231	2.231
Rmerge	0.146	0.595
Rmeas	0.149	0.641
Rpim	0.048	0.235
Number of reflections	9229	900
<I/σ(I)>	13.78	2.44
Completeness [%]	99.6	97.91
Redundancy	9.6	7.3
CC(1/2)	0.997	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10% PEG 8000, 0.1M HEPES pH 7.5, 8% Ethylene glycol, 0.04% Cortisone, 0.04% Epinephrine, 0.04% Protoporphyrin disodium salt, 0.04% Pyridoxine, 0.04% Thymidine monophosphate