7BRL

Atrial Natriuretic Peptide Receptor complexed with Deletion mutant of rat Atrial Natriuretic Peptide[4-17,23]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-28
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	100.273, 100.273, 270.415
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.517 - 3.200
R-factor	0.1995
Rwork	0.198
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp4
RMSD bond length	0.007
RMSD bond angle	0.822
Data reduction software	HKL-2000 (716.1)
Data scaling software	HKL-2000 (716.1)
Phasing software	MOLREP (11.2.08)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.200	3.200
Rmerge	0.048	0.373
Number of reflections	24713	2096
<I/σ(I)>	27.6	3.5
Completeness [%]	97.4
Redundancy	23.1
CC(1/2)	0.990	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	Sodium malonate, MES