7BRI

Atrial Natriuretic Peptide Receptor complexed with Dendroaspis Natriuretic Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-19
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 61
Unit cell lengths	99.597, 99.597, 262.467
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.711 - 2.450
R-factor	0.2018
Rwork	0.201
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp4
RMSD bond length	0.006
RMSD bond angle	0.829
Data reduction software	HKL-2000 (716.1)
Data scaling software	HKL-2000 (716.1)
Phasing software	MOLREP (11.2.08)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.520
High resolution limit [Å]	2.450	2.450
Rmerge	0.053	0.255
Number of reflections	51990	4457
<I/σ(I)>	30	4.6
Completeness [%]	96.3
Redundancy	22.1
CC(1/2)	0.990	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	Sodium malonate, MES