7BQW

Crystal structure of Methionine gamma-lyase from Fusobacterium nucleatum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-01-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9789
Spacegroup name	P 31 2 1
Unit cell lengths	95.205, 95.205, 302.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.750 - 2.500
R-factor	0.2172
Rwork	0.214
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dx5
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	56011	55899
<I/σ(I)>	23.77
Completeness [%]	99.8
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% PEG 5000, 0.2M sodium formate, 0.1M BICINE pH 8.5