7BQG

Complex structure of SAV1 and Dendrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	38.803, 43.084, 99.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.693 - 1.550
R-factor	0.181655015371
Rwork	0.178
R-free	0.21658
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bqf
RMSD bond length	0.006
RMSD bond angle	1.031
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	11875	599
<I/σ(I)>	39.1
Completeness [%]	99.6
Redundancy	12.8
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2% w/v PEG 2000 MME and 100 mM BIS-TRIS propane (pH 9.0)