7BQF

Dimerization of SAV1 WW tandem

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 31 2 1
Unit cell lengths	46.715, 46.715, 83.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.717 - 1.700
R-factor	0.198462671177
Rwork	0.196
R-free	0.21988
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ysb 2dwv
RMSD bond length	0.007
RMSD bond angle	0.971
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	11842	602
<I/σ(I)>	48.6
Completeness [%]	100.0
Redundancy	18.9
CC(1/2)	0.950	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2% v/v 1,4-Dioxane, 20% w/v PEG 3350 and 100 mM Tris-HCl (pH 8.0)