7BQ9
Crystal structure of ASFV p15
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.071460 |
Spacegroup name | P 21 3 |
Unit cell lengths | 116.737, 116.737, 116.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.658 - 2.612 |
R-factor | 0.1849 |
Rwork | 0.181 |
R-free | 0.21810 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.610 |
Number of reflections | 31107 | 4975 |
<I/σ(I)> | 30.73 | |
Completeness [%] | 99.6 | |
Redundancy | 40.2 | |
CC(1/2) | 1.000 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2.0 M Ammonium sulfate, 0.08M BIS-TRIS propane PH 7.0, 0.22M Sodium malonate PH 7.0, 0.02M HEPES PH7.0, 0.1% v/v Jeffamine ED-2001 PH 7.0 |