7BQ9

Crystal structure of ASFV p15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.071460
Spacegroup name	P 21 3
Unit cell lengths	116.737, 116.737, 116.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.658 - 2.612
R-factor	0.1849
Rwork	0.181
R-free	0.21810
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.600	2.610
Number of reflections	31107	4975
<I/σ(I)>	30.73
Completeness [%]	99.6
Redundancy	40.2
CC(1/2)	1.000	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2.0 M Ammonium sulfate, 0.08M BIS-TRIS propane PH 7.0, 0.22M Sodium malonate PH 7.0, 0.02M HEPES PH7.0, 0.1% v/v Jeffamine ED-2001 PH 7.0