7BQ7

Crystal structure of 2019-nCoV nsp16-nsp10 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-03-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9785
Spacegroup name	P 31 2 1
Unit cell lengths	170.156, 170.156, 52.218
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.220 - 2.370
R-factor	0.1812
Rwork	0.180
R-free	0.20850
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	XDS
Refinement software	PHENIX (1.18rc3_3805)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.080	2.500
High resolution limit [Å]	2.370	2.370
Number of reflections	35306	5118
<I/σ(I)>	18.8
Completeness [%]	99.7
Redundancy	18.5
CC(1/2)	0.998	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6