7BPR

Crystal structure of human TEX101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0717
Spacegroup name	P 1
Unit cell lengths	48.053, 49.480, 57.202
Unit cell angles	81.70, 87.84, 61.41

Refinement procedure

Resolution	43.030 - 1.950
R-factor	0.1931
Rwork	0.191
R-free	0.23450
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.620
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.030	42.990	2.000
High resolution limit [Å]	1.950	8.940	1.950
Rmerge	0.084	0.041	0.870
Rmeas	0.091	0.045	0.939
Rpim	0.034	0.017	0.351
Total number of observations		2567	14864
Number of reflections	32254	349	2134
<I/σ(I)>	17.8	67.5	2.3
Completeness [%]	96.6	98.9	88.7
Redundancy	7.1	7.4	7
CC(1/2)	0.999	0.999	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	27% (w/v) PEG3350, 0.1 M MES pH 6.3, 0.2 M potassium iodide