7BP6

Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	40.114, 62.282, 66.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.460 - 1.580
R-factor	0.1641
Rwork	0.162
R-free	0.20020
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.666
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.640
High resolution limit [Å]	1.580	1.580
Rmerge	0.066	0.428
Rmeas	0.078	0.382
Rpim	0.023	0.135
Number of reflections	23604	2302
<I/σ(I)>	28.5	4.4
Completeness [%]	99.7
Redundancy	9.6
CC(1/2)		0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2 M sodium nitrate, 0.1 M Bis-Tris propane and 20% (w/v) PEG 3350 (pH 8.5)