7BP5
Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.211, 62.747, 68.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.940 - 1.900 |
| R-factor | 0.1702 |
| Rwork | 0.168 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.046 | 0.788 |
| Rmeas | 0.070 | 0.548 |
| Rpim | 0.021 | 0.214 |
| Number of reflections | 38099 | 3778 |
| <I/σ(I)> | 29.1 | 2.5 |
| Completeness [%] | 99.8 | |
| Redundancy | 9.3 | |
| CC(1/2) | 0.212 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M sodium acetate, 20% PEG 3350 |
