7BP5

Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	40.211, 62.747, 68.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.940 - 1.900
R-factor	0.1702
Rwork	0.168
R-free	0.21600
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.788
Rmeas	0.070	0.548
Rpim	0.021	0.214
Number of reflections	38099	3778
<I/σ(I)>	29.1	2.5
Completeness [%]	99.8
Redundancy	9.3
CC(1/2)		0.212

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.2 M sodium acetate, 20% PEG 3350