7BP4

Structural insights into nucleosome reorganization by NAP1-RELATED PROTEIN 1 (NRP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	61.748, 62.248, 130.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.930 - 2.100
R-factor	0.1879
Rwork	0.186
R-free	0.22620
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.676
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.106	0.611
Rmeas	0.133	0.569
Rpim	0.042	0.215
Number of reflections	29973	2903
<I/σ(I)>	17.5	2.4
Completeness [%]	99.8
Redundancy	9.4
CC(1/2)	0.997	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	290	0.7 M ammonium dihydrogen phosphate, 0.07 M sodium citrate and 30% (v/v) glycerol (pH 5.6)