7BON
Crystal structure of recombinant horse spleen apo-R52C/E56C/R59C/E63C-Fr
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 181.739, 181.739, 181.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.450 - 1.480 |
R-factor | 0.16556 |
Rwork | 0.164 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dat |
RMSD bond length | 0.015 |
RMSD bond angle | 2.049 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.450 | 1.533 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmeas | 0.080 | 0.309 |
Rpim | 0.034 | 0.131 |
Number of reflections | 43077 | 4221 |
<I/σ(I)> | 12.76 | |
Completeness [%] | 99.5 | 99.91 |
Redundancy | 5.5 | |
CC(1/2) | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulphate, Cadmium sulphate |