7BOL

ubiquitin-conjugating enzyme, Ube2D2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2016-05-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	48.374, 52.185, 63.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.476 - 1.797
R-factor	0.1939
Rwork	0.193
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2esk
RMSD bond length	0.007
RMSD bond angle	0.919
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.797
Rmerge	0.072
Number of reflections	14967
<I/σ(I)>	26.3
Completeness [%]	96.8
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.5 M Ammonium sulfate 0.1 M Sodium citrate pH 5.6 1.0 M Lithium sulfate