7BNX
Archeal holliday junction resolvase from Thermus thermophilus phage 15-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.7701 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.710, 104.170, 84.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.950 - 2.551 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7bgs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.698 | |
| Number of reflections | 14269 | 1682 |
| <I/σ(I)> | 47.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 50.9 | |
| CC(1/2) | 1.000 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 1.8 M Ammoniumsulphate and 0.1 M sodiumacetate pH 4.2 |
