7BNV

Crystal Structure of the SARS-CoV-2 Receptor Binding Domain in Complex with Antibody ION-300

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.96861
Spacegroup name	P 43
Unit cell lengths	74.861, 74.861, 143.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.230 - 2.350
R-factor	0.2276
Rwork	0.224
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
RMSD bond length	0.004
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (v1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.230	2.434
High resolution limit [Å]	2.350	2.350
Number of reflections	32467	3234
<I/σ(I)>	16.34
Completeness [%]	99.1
Redundancy	1.9
CC(1/2)	0.997	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	16% PEG3350 0.3 M potassium citrate tribasic