7BNT

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikD with a predicted ancestral HMA domain of Pik-1 from Oryza spp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9700
Spacegroup name	P 41 21 2
Unit cell lengths	119.486, 119.486, 35.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.810 - 1.320
R-factor	0.1472
Rwork	0.145
R-free	0.18390
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5a6w
RMSD bond length	0.011
RMSD bond angle	1.590
Data reduction software	Aimless (0.7.4)
Data scaling software	DIALS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.810	59.810	1.340
High resolution limit [Å]	1.320	7.230	1.320
Rmerge	0.068	0.052	2.201
Rmeas	0.070	0.055	2.285
Rpim	0.018	0.015	0.605
Number of reflections	59464	462	2862
<I/σ(I)>	16.7
Completeness [%]	96.3	99.7	94.1
Redundancy	14.6	12.3	13.9
CC(1/2)	0.999	0.998	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	NULL