7BN3

Crystal structure of C-terminal domain of PABPC1 in complex with Nucleoprotein from Human Coronavirus 229E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.003
Spacegroup name	C 2 2 21
Unit cell lengths	63.509, 150.065, 65.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 1.930
R-factor	0.1949
Rwork	0.193
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kuj
RMSD bond length	0.009
RMSD bond angle	1.449
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.630	2.000
High resolution limit [Å]	1.930	1.930
Number of reflections	23731	2268
<I/σ(I)>	12.7
Completeness [%]	99.1
Redundancy	13.2
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M MES sodium salt pH-6.5, 1.8 M Ammonium Sulfate