7BN1

Clathrin heavy chain N-terminal domain complexed with peptide from Protein mu-NS of Reovirus type 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	137.533, 128.932, 78.100
Unit cell angles	90.00, 115.50, 90.00

Refinement procedure

Resolution	89.430 - 1.970
R-factor	0.1854
Rwork	0.184
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c9i
RMSD bond length	0.009
RMSD bond angle	1.540
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.430	2.110
High resolution limit [Å]	1.970	1.970
Number of reflections	61571	3080
<I/σ(I)>	9.1
Completeness [%]	93.3
Redundancy	3.5
CC(1/2)	0.991	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	30% PEG 550 MME; PEG 20K, 0.12M Monosaccharides (D-Glucose; D-Mannose; D-Galactose; L-Fucose; D-Xylose; N-Acetyl-D-Glucosamine) and 0.1M Sodium HEPES; MOPS (acid) pH-7.5