7BN1
Clathrin heavy chain N-terminal domain complexed with peptide from Protein mu-NS of Reovirus type 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.533, 128.932, 78.100 |
| Unit cell angles | 90.00, 115.50, 90.00 |
Refinement procedure
| Resolution | 89.430 - 1.970 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.540 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.430 | 2.110 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 61571 | 3080 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 93.3 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.991 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 30% PEG 550 MME; PEG 20K, 0.12M Monosaccharides (D-Glucose; D-Mannose; D-Galactose; L-Fucose; D-Xylose; N-Acetyl-D-Glucosamine) and 0.1M Sodium HEPES; MOPS (acid) pH-7.5 |
