7BMV
p62PH in cesium chloride (0.25 M CsCl in protein buffer and cryo protectant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7712 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.740, 102.300, 75.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.270 - 1.900 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.22330 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.826 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.270 | 42.270 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.093 | 0.055 | 1.420 |
| Rmeas | 0.095 | 0.057 | 1.452 |
| Rpim | 0.019 | 0.012 | 0.296 |
| Total number of observations | 3124 | 15642 | |
| Number of reflections | 12135 | 140 | 692 |
| <I/σ(I)> | 21.6 | 62.1 | 2.1 |
| Completeness [%] | 97.8 | 99.5 | 92.3 |
| Redundancy | 23.7 | 22.3 | 22.6 |
| CC(1/2) | 0.999 | 0.999 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.6 M potassium chloride, 15 % (w/v) PEG 4000 |
