7BM8
Crystal structure of the C-terminally truncated chromosome-partitioning protein ParB from Caulobacter crescentus complexed with CTP-gamma-S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.461, 56.076, 71.360 |
| Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
| Resolution | 68.810 - 2.730 |
| R-factor | 0.2493 |
| Rwork | 0.248 |
| R-free | 0.28390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t1f |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.188 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.590 | 70.590 | 2.860 |
| High resolution limit [Å] | 2.730 | 9.050 | 2.730 |
| Rmerge | 0.195 | 0.063 | 1.210 |
| Rmeas | 0.212 | 0.069 | 1.357 |
| Rpim | 0.083 | 0.028 | 0.599 |
| Total number of observations | 92266 | 2561 | 8473 |
| Number of reflections | 14516 | 432 | 1756 |
| <I/σ(I)> | 5.4 | 13.5 | 1.2 |
| Completeness [%] | 98.8 | 99.7 | 91.4 |
| Redundancy | 6.4 | 5.9 | 4.8 |
| CC(1/2) | 0.991 | 0.991 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | NULL |
