7BM2

Crystal structure of the DNA-binding protein RemA from Geobacillus thermodenitrificans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.966000
Spacegroup name	P 2 3
Unit cell lengths	106.910, 106.910, 106.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.800 - 2.290
R-factor	0.1802
Rwork	0.178
R-free	0.22320
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.328
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.800	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.194
Number of reflections	18626	1822
<I/σ(I)>	19.48	1.18
Completeness [%]	99.7
Redundancy	36.8
CC(1/2)	0.990	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.6 M ammonium sulphate, 0.1 M citric acid pH 3.5, final pH 4