7BLL

Structure of SusD homologue BT3013 from Bacteroides thetaiotaomicron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	114.530, 65.830, 87.020
Unit cell angles	90.00, 95.83, 90.00

Refinement procedure

Resolution	56.970 - 1.760
R-factor	0.1645
Rwork	0.163
R-free	0.20010
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.119
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.570	1.800
High resolution limit [Å]	1.760	1.760
Number of reflections	372154	19950
<I/σ(I)>	15.5
Completeness [%]	99.4
Redundancy	5.9
CC(1/2)	0.994	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein at 14mg/ml 30% w/v PEG 4000, 0.1 M sodium citrate pH 5.5 and 0.2 M ammonium acetate