7BKX

Diploptera punctata inspired lipocalin-like Milk protein expressed in Saccharomyces cerevisiae

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 2
Unit cell lengths	51.610, 135.662, 39.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.600 - 2.350
Rwork	0.222
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nyq
RMSD bond length	0.013
RMSD bond angle	2.101
Data reduction software	xia2 (5.8.0258)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.612	2.390
High resolution limit [Å]	2.350	2.350
Rmeas	0.073	0.836
Number of reflections	12200	1224
<I/σ(I)>	18.1
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.998	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	15% of PEG MME 550, 100 mM MES pH 6.5, and 10 mM ZnSO4