7BKV
Endothiapepsin structure obtained at 100K with fragment AC39729 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.295, 73.492, 52.830 |
Unit cell angles | 90.00, 109.93, 90.00 |
Refinement procedure
Resolution | 42.580 - 1.240 |
R-factor | 0.1423 |
Rwork | 0.141 |
R-free | 0.16190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rsv |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.580 | 1.280 |
High resolution limit [Å] | 1.240 | 1.240 |
Rpim | 0.021 | 0.448 |
Number of reflections | 70862 | 4490 |
<I/σ(I)> | 19.22 | 1.51 |
Completeness [%] | 77.1 | |
Redundancy | 6.3 | |
CC(1/2) | 0.990 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 100 mM ammonium acetate, 100 mM sodium acetate pH 4.6 and 26 to 30% PEG 4000 |