7BKU
Endothiapepsin structure obtained at 100K with fragment JFD03909 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.286, 73.329, 52.756 |
Unit cell angles | 90.00, 109.69, 90.00 |
Refinement procedure
Resolution | 49.670 - 1.400 |
R-factor | 0.1534 |
Rwork | 0.152 |
R-free | 0.18020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rsv |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.670 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rpim | 0.044 | 0.735 |
Number of reflections | 59946 | 4212 |
<I/σ(I)> | 12.19 | 1.18 |
Completeness [%] | 94.4 | |
Redundancy | 6.7 | |
CC(1/2) | 0.990 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 100 mM ammonium acetate, 100 mM sodium acetate pH 4.6 and 26 to 30% PEG 4000 |