7BJQ
Crystal structure of RecJCdc45 from Methanothermobacter thermoautotroficus in complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.080, 69.501, 72.210 |
| Unit cell angles | 86.12, 69.26, 69.53 |
Refinement procedure
| Resolution | 67.380 - 2.700 |
| R-factor | 0.1997 |
| Rwork | 0.196 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tvv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.390 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 11350 | 3804 |
| <I/σ(I)> | 9.6 | 1.9 |
| Completeness [%] | 98.1 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.984 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M bis-Tris pH 5.5, 17% w/v PEG 10000, 1M NH4CH3CO2 |
