7BJK
Crystal structure of the chloroplastic Fe superoxide dismutase PAP9 from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 214.090, 83.010, 118.240 |
| Unit cell angles | 90.00, 115.76, 90.00 |
Refinement procedure
| Resolution | 48.200 - 2.250 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1unf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.894 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.108 | 0.779 |
| Number of reflections | 83998 | 5642 |
| <I/σ(I)> | 7.87 | 1.03 |
| Completeness [%] | 94.5 | 86 |
| Redundancy | 3.82 | 2.32 |
| CC(1/2) | 0.995 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350 from 15 to 19 %, 0.1 M BisTris pH 6.5, 0.2 M NaNO3 |
