7BIL
Crystal structure of helicase Pif1 from Thermus oshimai in complex with oligo GGTTTGGTTTGGTT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979150 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.052, 103.438, 251.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.020 - 2.210 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6s3m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.387 |
| Data reduction software | XDS (BUILT 20200131) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.783 | 2.250 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.077 | 1.126 |
| Rmeas | 0.083 | 1.217 |
| Rpim | 0.031 | 0.457 |
| Number of reflections | 404851 | 1459 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 99.6 | 51 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.999 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | Tris-HCl 0.1M NaF 0.03M NaI 0.03M NaBr 0.03M MES 0.03M PEG 4K 10% |
