7BHI
Crystal structure of RecJ-Cdc45 from Methanothermobacter Thermoautotrophicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.471, 55.010, 89.990 |
| Unit cell angles | 81.38, 86.36, 89.95 |
Refinement procedure
| Resolution | 88.790 - 2.700 |
| R-factor | 0.2326 |
| Rwork | 0.231 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tvv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.483 |
| Data reduction software | iMOSFLM |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.790 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 19511 | 2782 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 92.8 | 90.4 |
| Redundancy | 1.7 | 1.6 |
| CC(1/2) | 0.970 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Tris/HCl pH 8.5, 27% w/v PEG 4000, 0.2M MgCl2*6H20, 1mM MnCl2 |
